CD8 T Cell Response Maturation Defined by Anentropic Specificity and Repertoire Depth Correlates with SIVΔnef-induced Protection

نویسندگان

  • Sama Adnan
  • Arnaud D. Colantonio
  • Yi Yu
  • Jacqueline Gillis
  • Fay E. Wong
  • Ericka A. Becker
  • Michael Piatak
  • R. Keith Reeves
  • Jeffrey D. Lifson
  • Shelby L. O’Connor
  • R. Paul Johnson
چکیده

The live attenuated simian immunodeficiency virus (LASIV) vaccine SIVΔnef is one of the most effective vaccines in inducing protection against wild-type lentiviral challenge, yet little is known about the mechanisms underlying its remarkable protective efficacy. Here, we exploit deep sequencing technology and comprehensive CD8 T cell epitope mapping to deconstruct the CD8 T cell response, to identify the regions of immune pressure and viral escape, and to delineate the effect of epitope escape on the evolution of the CD8 T cell response in SIVΔnef-vaccinated animals. We demonstrate that the initial CD8 T cell response in the acute phase of SIVΔnef infection is mounted predominantly against more variable epitopes, followed by widespread sequence evolution and viral escape. Furthermore, we show that epitope escape expands the CD8 T cell repertoire that targets highly conserved epitopes, defined as anentropic specificity, and generates de novo responses to the escaped epitope variants during the vaccination period. These results correlate SIVΔnef-induced protection with expanded anentropic specificity and increased response depth. Importantly, these findings render SIVΔnef, long the gold standard in HIV/SIV vaccine research, as a proof-of-concept vaccine that highlights the significance of the twin principles of anentropic specificity and repertoire depth in successful vaccine design.

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Correction: CD8 T Cell Response Maturation Defined by Anentropic Specificity and Repertoire Depth Correlates with SIVΔnef-induced Protection.

This article was republished on April 15, 2015 to correct an error where the Table 1 legend was included in the main text of the article. The publisher apologizes for these errors, which were introduced during the typesetting process. Please download this article again to view the correct version. The originally published, uncorrected article and the republished, corrected article are provided ...

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عنوان ژورنال:

دوره 11  شماره 

صفحات  -

تاریخ انتشار 2015